Research Articles
Step-by-Step CRISPR-Cas9 Gene Knockout in Zebrafish: A Complete Protocol for Disease Modeling and Drug Discovery
This comprehensive guide details a complete, optimized protocol for generating stable knockout zebrafish lines using CRISPR-Cas9.
CRISPR-Cas9 Guide: Functional Validation of Genetic Variants in Disease Research and Drug Discovery
This comprehensive protocol provides researchers and drug development professionals with a step-by-step framework for using CRISPR-Cas9 to functionally validate genetic variants.
Complete Guide to CRISPR-Cas9 Gene Knockout in Zebrafish: Protocols, Troubleshooting, and Validation
This comprehensive guide provides researchers and drug development professionals with a detailed, step-by-step protocol for performing CRISPR-Cas9-mediated gene knockout in zebrafish.
CRISPR-Cas9 Functional Validation: From Genetic Variant to Biological Consequence in Drug Discovery
This article provides a comprehensive guide for researchers and drug development professionals on utilizing CRISPR-Cas9 for the functional validation of genetic variants.
From Phage Wars to Genetic Scissors: Unpacking the CRISPR-Cas9 Bacterial Immune System for Therapeutic Innovation
This article provides a comprehensive analysis of the CRISPR-Cas9 system, tracing its origins as a bacterial adaptive immune defense against bacteriophages to its revolutionary applications in genetic engineering and drug...
CRISPR vs. RNAi Screening: A Comprehensive Comparison of Sensitivity and Specificity for Functional Genomics
This article provides a detailed, comparative analysis of CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) and RNA interference (RNAi) screening technologies, focusing on their core differences in sensitivity and specificity.
CRISPR Functional Validation: A Comprehensive Guide to Confirming NGS Variants in Research & Drug Discovery
This article provides a comprehensive roadmap for researchers, scientists, and drug development professionals to functionally validate next-generation sequencing (NGS) findings using CRISPR-Cas genome editing.
A Comprehensive Guide to CRISPR Screening with NGS Readout: From Library Design to Data Analysis
This article provides a complete roadmap for implementing CRISPR screening with Next-Generation Sequencing (NGS) readout, tailored for researchers, scientists, and drug development professionals.
CRISPR vs RNAi Screening: Choosing the Right Functional Genomics Tool for Your Research
This comprehensive guide compares CRISPR and RNAi screening technologies, exploring their foundational principles, methodological workflows, optimization strategies, and comparative performance metrics.
CRISPR Screening: How Much Sequencing Depth Do You Really Need? A Data-Driven Guide for Researchers
This article provides a comprehensive guide to determining optimal sequencing depth for CRISPR knockout and activation screens.