Research Articles
Designing CRISPR Libraries: A Complete Guide to Knockout and Activation Screens for Functional Genomics
This comprehensive guide provides researchers and drug development professionals with a detailed framework for designing and implementing CRISPR library screens for gene knockout and activation.
CRISPR Knockout vs. RNAi/shRNA Screens: A Practical Guide to Sensitivity, Specificity, and Screen Selection for Genetic Discovery
This comprehensive guide for researchers and drug development professionals explores the critical distinctions between CRISPR-Cas9 knockout and RNAi/shRNA screening technologies.
A Step-by-Step CRISPR Knockout Screen Protocol: From Library Design to High-Confidence Hit Discovery
This comprehensive guide details a complete, optimized protocol for performing a high-throughput CRISPR-Cas9 knockout screen.
Validating CRISPR Knockouts: A Guide to Orthogonal RNAi Confirmation for Robust Gene Function Analysis
This article provides a comprehensive guide for researchers on employing RNAi as an orthogonal validation method for CRISPR-Cas9 knockout studies.
Precise Gene Silencing with CRISPRi: A Comprehensive Guide for Vertebrate Model Systems in Biomedical Research
This article provides a detailed exploration of CRISPR interference (CRISPRi) techniques specifically adapted for vertebrate model organisms, including zebrafish, mice, and organoids.
CRISPR Activation Screening: A Comprehensive Guide to Uncover Drug Resistance Mechanisms
This article provides researchers and drug development professionals with a detailed guide to using CRISPR activation (CRISPRa) screening for identifying genes that confer resistance to therapeutic agents.
CRISPR-Cas9 Pooled Screening Protocol: Comprehensive Guide to Optimization for Robust Genetic Discovery
This article provides a detailed roadmap for researchers, scientists, and drug development professionals to optimize CRISPR-Cas9 pooled screening protocols.
A Step-by-Step CRISPR-Cas9 Pooled Screening Protocol: From sgRNA Library Design to Hit Validation
This comprehensive guide provides researchers, scientists, and drug development professionals with a detailed, modern protocol for conducting a successful CRISPR-Cas9 pooled knockout screen.
Decoding the Genome: A Comprehensive Guide to CRISPR-Cas9 Knockout Screens
This guide provides researchers, scientists, and drug development professionals with a detailed exploration of CRISPR-Cas9 knockout screen principles.
CRISPR-Cas9 Functional Genomics: Revolutionizing Disease Modeling for Precision Medicine
This article provides a comprehensive guide for researchers and drug development professionals on leveraging CRISPR-Cas9 functional genomics for sophisticated disease modeling.